Determination of STAT Blood Gases and chemistries by perfusionsist Using the I-STAT Portable Clinical Analyzer
The policies and procedures contained in this manual are to be strictly followed. For addioonal information or assistance call Point of Care Testing.
Warning! Thie Policy of Universal Precautions must be followed during all laboratory procedures. This is mandated by OSHA.
PURPOSE OF TEST
The I-STAT Chemistry Analyzer is used in patient care areas:
- where results are needed urgently (e.g. intensive care unit/emergency room/operating room)
- where there is requirement to reduce the specimen size (e.g. nurseries)
- where patient treatment regimes require it ( e.g. dialysis or CPB).
Note: Only personnel with documented training on this instrument may operate it. Violations of these regulations of the policies and procedures that follow will result in the removal of the system from the patient care area until satisfactory remedial action is taken.
The I-STAT system incorporates comprehensive components needed to perform blood analysis at the point of care. Just 2-3 drops of fresh whole blood is all that is required, and the portable batter-operated analyzer displays quantitative test results in approximately 2 min. The system consists of the following: the cartridge, the analyzer, and a central data station.
PRINCIPLES OF THE TESTS
A single-use disposable cartridge contains a microfabricated sensor array, a calibrant solution, fluidics system, and a waste chamber. Sensors fir analysis of sodium potassium, chloride, ionized calcium, PpH, PCO2, PO2 urea nitrogen (BUN), glucose, and hematocrit are available in a variety of panls. A whole blood sample of approximately 2-4 drops is dispensed into the cartridge sample well.
A handheld analyzer into hich the blood-filled cartridge is placed for analysis automatically controls all functions of the testing cycle, including fluid movement within the cartridge