CP34 Bone Marrow Aspirate Concentrate

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CP34 Bone Marrow Aspirate Concentrate

 

WHAT IS IT ?

Bone Marrow Aspirate Concentrate “BMAC” is a minimally invasive autologous blood therapy procedure that uses the patient’s own stem cells in concentrated form to stimulate the healing process. Stem cells play a vital role in every healing process within the human body, especially for osteogenesis (bone formation) and angiogenesis (new blood vessel growth).

WHO IS IT FOR ?

As we age our ratio of stem cells to total cells decrease over time – which is why young people heal so much faster than elderly people. BMAC is effective because it concentrates the stem cells into a therapeutic range that mimics the natural healing process that occurs in a younger patient.

Many surgeons are using BMAC with aging patients in need of spinal fusions, hip revisions, AVN, non-union, fractures and other orthopedic injuries.

Additionally, BMAC is being utilized as a safe alternative option for chronic knee and back pain rather than having to undergo a more invasive surgery that can be traumatic, painful and require many months of rehabilitation.

HOW DOES IT WORK ?

Bone Marrow Aspirate Concentrate (BMAC) is prepared from bone marrow obtained from the iliac crest (hip) – where the highest concentration of stem cells in the body reside.

Once aspirated, the clinical technician concentrates and prepares the BMAC for the surgeon to apply into the surgical site. The bone marrow aspirate is placed into a centrifuge that separates and concentrates the stem cells, which becomes a gelatinous liquid that can be delivered to the surgical site through enriched bone grafts, percutaneous injections or topical spray.

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BACKGROUND

Recent approaches have sought to harness the potential of stem cells to regenerate bone that is lost as a consequence of trauma or disease. Bone marrow aspirate (BMA) provides an autologous source of osteoprogenitors for such applications. However, previous studies indicated that the concentration of osteoprogenitors present in BMA is less than required for robust bone regeneration. We provide further evidence for the importance of BMA enrichment for skeletal tissue engineering strategies using a novel acoustic wave-facilitated filtration strategy to concentrate BMA for osteoprogenitors, clinically applicable for intraoperative orthopedic use.

METHODS:

Femoral BMA from 15 patients of an elderly cohort was concentrated for the nucleated cell fraction against erythrocytes and excess plasma volume via size exclusion filtration facilitated by acoustic agitation. The effect of aspirate concentration was assessed by assays for colony formation, flow cytometry, multilineage differentiation and scaffold seeding efficiency.

RESULTS:

BMA was filtered to achieve a mean 4.2-fold reduction in volume with a corresponding enrichment of viable and functional osteoprogenitors, indicated by flow cytometry and assays for colony formation. Enhanced osteogenic and chondrogenic differentiation was observed using concentrated aspirate and enhanced cell-seeding efficiency onto allogeneic bone graft as an effect of osteoprogenitor concentration relative specifically to the concentration of erythrocytes in the aspirate.

CONCLUSIONS:

These studies provide evidence for the importance of BMA nucleated cell concentration for both cell differentiation and cell seeding efficiency and demonstrate the potential of this approach for intraoperative application to enhance bone healing.

Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

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